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Old 01-16-2015, 12:36 AM   #16
Simone78
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Location: Basel (Switzerland)

Join Date: Oct 2010
Posts: 205
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Quote:
Originally Posted by dagarfield View Post
We've not yet tried this, but I'm considering it -- it sure would be nice to barcode during the Tagmentation step rather than the PCR step....if someone tries this, there's a beer in it for you at the next meeting.....
I am not doing ATAC-seq but I might help you anyway. Instead of spending a lot of money for few ul of Tn5 from Epicentre you could just order the plasmid from Addgene (ID: 60240) and make it yourself. Then you could all the adaptors you like!
We have recently published a paper about it, take a look!
http://www.ncbi.nlm.nih.gov/pubmed/25079858
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