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Old 07-07-2015, 12:14 PM   #19
intregen
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Location: Baltimore, MD

Join Date: Jul 2015
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Quote:
Originally Posted by dagarfield View Post
It seems to work just fine: If you can see your post-PCR product on a gel, you'll have a fine library (in our experience). Some of the runs have more mtDNA reads than you would expect from, say, DNase-Seq, but this is something many have reported (and is seen in the data from the original paper).

In our experience, the most significant issue is simply making sure that we don't lose our pellet of cells/nuclei during the process.

I should note that we're only using the buffer recipe you linked to wash the cells. The reaction itself still takes place in the commercial buffer. Probably going to stay that way unless someone has a method for making Tn5 independent of the kit.....
Has anyone used the homemade TD buffer for the ATAC reaction and not just for washes? Does it work just as well?
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