Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Command line blast with remote option

    Hello,
    I am trying to run blastn from my command line with the -remote option, and I get a proxy error as

    Error: (311.26) SOCK#1000[6]@130.14.29.110:80: [SOCK::Connect] Failed pending connect(): Closed {error=111,Connection refused}
    Error: (303.7) [URL_Connect] Failed to connect to www.ncbi.nlm.nih.gov:80: Closed
    Error: (311.26) SOCK#2000[6]@130.14.29.110:80: [SOCK::Connect] Failed pending connect(): Closed {error=111,Connection refused}
    ...

    Any idea how to fix this please?
    Thanks

  • #2
    It is a network problem, so it could go away if you retry at a less busy time. Can you use BLAST from your browser? Is there a proxy in the way? If so, you might need to talk to your network administrator.

    Comment


    • #3
      It is a proxy error, I think. I was wondering if there is a proxy option in blastn itself. I can run it from the browser. But I can also run it directly from applications like clcBio. So the configuration can be done - just not sure how to do it for blastn.
      Am getting my network people to look into it also..
      Thanks so much!

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin


        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
        Yesterday, 07:01 AM
      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      39 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      41 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      35 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      55 views
      0 likes
      Last Post seqadmin  
      Working...
      X