I don't know if I would say your Bioanalyzer image is inaccurate. Your agarose gel is overloaded and not run very far which will result in larger fragments running faster then they should. Your average fragment size appears to be 600 bp by intensity which means on a molar scale it would be less. The smaller inserts probably cluster more efficiently (I am just guessing here). So no in your case I do not think the agarose gel is more accurate then the Bioanalyzer.
Another explanation is you may have some over amplification issues as well, which result in the fragments running bigger then they are. If you combine this with the overloaded agarose gel making them run slower then they are, it makes the agarose gel look more accurate. The end result is the two artifacts canceling each other out. While ok for you in this case, it's not something I would want to rely on.
Another explanation is you may have some over amplification issues as well, which result in the fragments running bigger then they are. If you combine this with the overloaded agarose gel making them run slower then they are, it makes the agarose gel look more accurate. The end result is the two artifacts canceling each other out. While ok for you in this case, it's not something I would want to rely on.
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