Hi,
I used the Covaris to shear gDNA for Illumina libraries, but something went wrong because instead of the expected 500 bp fragments I got extensive (and pretty random) degradation. I am attaching the results from the bioanalyzer: sample 4 is a + control and looked fine, but I had to manually set high and low marker peaks for the remaining 3 samples. S1 and S2, in particular look really degraded. However, they seem to have enough 200-300 bp fragments that might be recovered with a size selection. Has anyone ever successfully built and sequenced illumina libraries from similarly over-fragmented gDNA?
Thank you in advance!
I used the Covaris to shear gDNA for Illumina libraries, but something went wrong because instead of the expected 500 bp fragments I got extensive (and pretty random) degradation. I am attaching the results from the bioanalyzer: sample 4 is a + control and looked fine, but I had to manually set high and low marker peaks for the remaining 3 samples. S1 and S2, in particular look really degraded. However, they seem to have enough 200-300 bp fragments that might be recovered with a size selection. Has anyone ever successfully built and sequenced illumina libraries from similarly over-fragmented gDNA?
Thank you in advance!
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