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Old 11-25-2009, 09:42 AM   #3
Hamid
Senior Member
 
Location: San Diego, CA

Join Date: Sep 2009
Posts: 105
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Hi Andy,

I do have a few questions:
1. Which tubes are you using for the processing of your 0.5ml samples?
2. What is the cell number you are using in the 0.5ml sample?
3. Are you preparing the nuclei prior to shearing?
4. What is the constituents of the shearing buffer?
5. What is the protocol you are using for preparing your cells for ChIP?
6. Did you carryout a time course shearing to determine the optimal shearing time?

We do have a protocol for chromatin shearing. It is located at http://covarisinc.com/pdf/pn_400066.pdf. please take a look, and let me know if you have any other questions. Please feel free to contact me directly.

Thank you.

hamid
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