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Old 11-27-2009, 10:01 AM   #5
Hamid
Senior Member
 
Location: San Diego, CA

Join Date: Sep 2009
Posts: 105
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Hi Andy,

I have definitely identified the problem you are having.
Please realize that our sample vessels are an integral part of our acoustic technology. We have invested a significant energy and time in their development to ensure that they do not interfere in the delivery of controlled acoustic energy to your samples. The use of the polypropylene tubes are not recommended for any of our shearing protocols requiring high energy delivery. The reason for that is that polypropylene, and polystyrene absorb as well as deflect a significant amount of the acoustic energy during high energy processing. This translates to heating of the tube, which in turn heats your sample. The absorption and deflection also greatly reduce the energy delivery to your samples.
As suggested in the protocol, http://covarisinc.com/pdf/pn_400066.pdf, please use either the TC12, or TC13 tubes depending on your sample volume. These glass tubes are specifically designed and validated for efficient delivery of controlled acoustic energy to your samples.

I also wanted to note that the protocols we have optimized and validated for shearing of naked DNA, are not transferable or usable for the shearing of chromatin.

Thank you

hamid
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