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Old 05-28-2015, 12:00 PM   #1
Smriti
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Location: California

Join Date: Mar 2011
Posts: 40
Default Is Nextera useful for Pac bio in future?

Hi There,

I am working with Nextera workflow. I was wondering if Nextera library prep can be somehow extended to Pac bio platform. My understanding is Nextera insert size is much smaller than what Pac bio can sequence. Is there a way Nextera transposon can fragment a bigger size insert, add adapters and then it gets sequenced on Pac bio.

This way, we can use advantage of both the technologies, low imput starting material and sequencing it real-time.

Any suggestions?
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