View Single Post
Old 05-28-2015, 09:27 PM   #2
SNPsaurus
Registered Vendor
 
Location: Eugene, OR

Join Date: May 2013
Posts: 521
Default

I suspect that if you were to reduce the amount of transposase compared to DNA then you would see an increase in the bias of the fragmentation, as the preferred sites would use up the low amount of reagent.

Nextera is nice because it allows you to skip end-repair and ligation of adapters. But if you want to make really long DNA fragments, it is probably preferable to just end repair and ligate and not add a fragmentation step.
__________________
Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com
SNPsaurus is offline   Reply With Quote