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Old 05-28-2015, 10:14 PM   #3
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232
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It can be done in two ways. One is to do tagmentation with more input DNA or reduced enzyme to obtain large tagmented DNA for amplification as suggested by SNPsaurus. Other is to tagment DNA as before but fill in the gap and end repair without amplification. One also can introduce size selection either way. Nextera mate pair tagmentation system also can be used but it is less cost effective.
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