Thread: Qiime
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Old 04-14-2016, 06:14 AM   #2
fanli
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Location: California

Join Date: Jul 2014
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The next step is to demultiplex your joined reads using a separate barcode file. The barcode sequences should match those in your mapping file.

see http://nbviewer.jupyter.org/github/b...tutorial.ipynb
Code:
split_libraries_fastq.py -o slout/ -i forward_reads.fastq.gz -b barcodes.fastq.gz -m map.tsv
you can replace forward_reads.fastq.gz with the fastq file output from join_paired_ends.py
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