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Old 12-18-2016, 05:48 PM   #2
Brian Bushnell
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Location: Walnut Creek, CA

Join Date: Jan 2014
Posts: 2,707

It looks to me like low library complexity due to overamplification. It's hard to say, though. Note that there is a peak at ~16x. In order to see it, you have to rotate the image to the left by 45 degrees... the fact that it is such a weak peak indicates a very wide spread of coverage, indicative of overamplification, or an extremely high error rate. Or severe contamination, which can also be a problem with low-input amplified libraries. Do the reads generally BLAST to related insects?
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