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Old 01-16-2017, 07:06 AM   #9
mohande
Junior Member
 
Location: Austria

Join Date: Jan 2017
Posts: 1
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Hi all,

I am trying to shear chromatin using Covaris S. I have tested 6 different conditions on Genomic DNA first and what it works for me was (20%, 10, 200, 15 min), but when I apply to chromatin it gives me a very large size range. I have used 12x24 tubes, not the 130 ul ones.

Here are 6 conditions I have applied to chromatin:
duty cycle 20%, 20%, 20%, 20%, 20%, 20%
intensity 10, 10,10,10,10,10
duty per bust 500,200, 200, 200, 200, 200
time (min) 30, 20,30,40,50

Can anybody tell me how can I shear my chromatin in the range on 200-700 bp.

Cheers :-)

ps: if that helps I am using this tubes:
http://covaris2.corecommerce.com/Con...m-100-p43.html

Last edited by mohande; 01-16-2017 at 07:56 AM.
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