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Old 05-04-2018, 08:42 AM   #6
r.rosati
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Location: Brazil

Join Date: Aug 2015
Posts: 92
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We have a 4200 and a very proficient student of mine tried to use it (per Thermo protocol re. Bioanalyzer) to quantify RNAseq libraries, but this didn't prove very reliable; she'd get a very unbalanced number of reads when she mixed libraries in a chip. Quantifying the same samples via qPCR gave much more reliable results. I don't blame the 4200 per se, though; perhaps the issue was uneven fragment lengths by our part. Anyways, qPCR proved to be less affected by experimental variation and that's what we use now.
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