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Old 09-09-2014, 10:41 PM   #4
shimbalama
bioinformatics-help.com
 
Location: Adelaide

Join Date: Jul 2014
Posts: 9
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Thanks Brian.

I do all that. What I am trying to do is QC on the negative var calls. So every base in every gene of interest (GOI).

What I am interested in is the mean read depth of every GOI that comes off my machine and whether it is significantly different to the mean depth I have defined as 'gold'. So the question is about statistical analysis only.
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