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Old 07-27-2018, 10:26 PM   #47
kokyriakidis
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Location: Thessaloniki, Greece

Join Date: Jul 2018
Posts: 12
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Hello! I have RNA-Seq data and I am processing them with BBtools for adapter trimming, quality trimming, contaminant filtering, error correction. I want to use these data as support in denovo genome assembly with Abyss, which has this ability. Should I normalize them? What is the target number that I should set in bbnorm? Can someone explain how I calculate the right number?

Last edited by kokyriakidis; 07-28-2018 at 12:38 AM.
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