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I started working on whole genome sequencing and exome sequencing with Illumina HiSeq 2000 and also with 454 sequencer. Can anybody please help me to understand the data analysis of the sequenced reads????
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If you'd like answers to such a question, you should start by posting in the correct forum. Then, explain what you want to do with the reads. You're query can be condensed to "I ran an experiment; how do I analyze it?", and nobody can really help you without some real detail.Originally posted by rd69 View Post -
Anyone remember the days were you just dumped 3 volumes of Maniatis on a student desk and turned around... probably not.Originally posted by rd69 View PostComment
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Moved this to a new thread...and I really encourage the original poster to read the following:
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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