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Old 07-21-2011, 06:07 AM   #6
Location: cinci

Join Date: Apr 2010
Posts: 66

Thanks for explaining I like to clarify to myself. Is the reason for the replicates to account for the bias created by the enrichment and other experiment bias? or they are different replicates? I want to clarify this point. I have concern in my data that there may be false positive DE caused by amplification bias. In other words, I am concerned that I may be detecting preferential PCR amplification instead of real expression of certain amplicons by the nature of the enzymatic approach and depending on sequence of amplicons. Just curious thought.
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