Hello!!
I'm quite new in NGS and I got these fastq files (read1 and read2) from illumina nextseq 500.
Now the problem is that in somehow the two file are not aligned, I mean, I checked for the coordinates in read1 and read2 on the same row and it appears that sometimes are not the same!
Since I'm quite new, this can be possible? or anyway is there any tools or bash script that can help me in the alignment of these files?
Hope seriously in somebody that can help me!
I'm quite new in NGS and I got these fastq files (read1 and read2) from illumina nextseq 500.
Now the problem is that in somehow the two file are not aligned, I mean, I checked for the coordinates in read1 and read2 on the same row and it appears that sometimes are not the same!
Since I'm quite new, this can be possible? or anyway is there any tools or bash script that can help me in the alignment of these files?
Hope seriously in somebody that can help me!
Comment