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Old 02-22-2016, 10:09 AM   #2
Location: United States

Join Date: May 2014
Posts: 40

Hi Jakob,

What method are you using to extract your RNA and how are you storing it (i.e. buffer, conditions, etc.)? If your extraction is yielding high-purity, intact RNA and both sets are stored and processed under identical conditions then the issue is likely caused by environmental contamination by RNases. Can you post representative traces from your samples, both before and after Ribo-Zero treatment? Also, what Ribo-Zero kit are you currently using and can you comment on the type of culture (i.e. single-species vs. co-culture)?
adam.geber is offline   Reply With Quote