Hi all,
I used the Bioo Scientific kit to make my barcoded ChIP-Seq libraries. The signal by PCR was similar across all 3 input control samples under different conditions. However, they became so different after I made the libraries. Strangely, my sample libraries have maintained specific enrichment of target sites. The bioanalyzer results look good. It seems to be a selection during the library prep? Has anyone encountered this problem?
Any suggestions are welcome!
Thank you in advance!
I used the Bioo Scientific kit to make my barcoded ChIP-Seq libraries. The signal by PCR was similar across all 3 input control samples under different conditions. However, they became so different after I made the libraries. Strangely, my sample libraries have maintained specific enrichment of target sites. The bioanalyzer results look good. It seems to be a selection during the library prep? Has anyone encountered this problem?
Any suggestions are welcome!
Thank you in advance!
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