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Old 03-24-2014, 01:33 PM   #56
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Location: Norway

Join Date: Aug 2013
Posts: 266

Thank you!

Another question, why is it that the absolute expression levels of genes is not important when doing GAGE/GOseq/DAVID etc.?

If you have a new cell type/tissue or even a species, how do you go about doing GAGE if you do NO COMPARISONS, only you want to get familiar with this new transcriptome?
You only have set of genes that expressed (including CPM/FPKM values), and you also know which genes thats not expressed.

Can you use this information? Basically, you just use one condition, and the expression values of the genes expressed.
sindrle is offline   Reply With Quote