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Old 07-29-2015, 03:43 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232

Bioanalyzer issues can be checked by looking at ladder run. If ladder looks OK then it is less likely to be instrument related. One possibility is carryover of beads during collection of purified DNA. This can be fixed by putting eluted samples back on magnet and recollecting. By the way, if the top sample is final RNA library (not SPIA amplified cDNA) it looks odd because of distinct bands.
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