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Old 07-30-2015, 07:00 AM   #3
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Location: Baltimore, MD

Join Date: Jul 2015
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Originally Posted by nucacidhunter View Post
Bioanalyzer issues can be checked by looking at ladder run. If ladder looks OK then it is less likely to be instrument related. One possibility is carryover of beads during collection of purified DNA. This can be fixed by putting eluted samples back on magnet and recollecting. By the way, if the top sample is final RNA library (not SPIA amplified cDNA) it looks odd because of distinct bands.
The ladder was fine, there were strong peaks for all of the standards. Thanks for the magnet suggestion, if I do anything more with the samples I'll keep them on a magnet for a while before pipetting them.
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