Has anyone automated any or all of the Illumina library prep steps on a robot? Beckman, Tecan, Hamilton? Any help will be appreciated. Thanks
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Same here
Looking for Library prep automation to increase throughput and eliminate variations caused by different users. In addition to the automation mentioned above from BIG_SNP, are there any other new instrumentation for full automation, say with thermal blocks?
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Originally posted by Michael L. Altshuler View PostSPRIworks Fragment Library System I and II are claimed to be the walk away automates for the library preparation (Beckman Genomics). I would like to know what they actually do, especially the start point (blood?) and endpoint (Adaptor Ligation???) for Illumina library
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We are currently trying to automate as much of the set up as possible. We are using DNA fragmentase to fragment the DNA and think we can automate a number of the subsequent ligation and adapter annealing steps, although this involves manually placing plates onto PCR blocks. We are going to be using the ampure xp for the PCR clean up steps and dont envisage that being problematic. Finally we are using nimblegen sequence capture in solution and have a protocol for that but we have not run any samples yet. We are going to use a beckman Fx for the pre-PCR steps and a beckman Nx for the post-PCR steps and we are aiming to run a test batch of samples in the next 2 weeks once all our reagents arrive. I'd be happy to pass on details when we have an optimised pipeline.
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Hi, I can confirm what James said above. The Sanger use a Biomek FX and a Biomek NX to automate their library prep for the Illumina. Beckman also have another site in the UK automating 96 library preps at a time on the Biomek FX with qPCR set up done on a Biomek 3000.
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