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Old 07-08-2013, 06:41 AM   #5
Location: Lyon, France

Join Date: May 2012
Posts: 12

Dear Aan,

Here are the settings we're using, on a Covaris S220; we usually got a peak size at 160bp within a range from 150bp to 180bp.

Peak power 175
Duty factor 10 100s
Cycles/burst 100

These three steps repeated 6 times. This is basically the same program i'm using for blood DNA but with a longer shearing time per cycle (100s versus 60s for blood DNA).

Hope it'll help,
durandg is offline   Reply With Quote