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Old 05-16-2012, 10:10 AM   #2
Hamid
Senior Member
 
Location: San Diego, CA

Join Date: Sep 2009
Posts: 105
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Hi Durandg,

Since you are starting with DNA that is already smaller in size than intact genomic DNA, you can shear down to the size range of interest on your S220, by using the same setting you currently use to shear genomic DNA. The only difference will be that you will have to extend the processing time. My suggestion is that you carry out a time course of up to 50%beyond the time you use for shearing genomic DNA. for example, if your setting requires a 10 minute processing time for genomic DNA, you should try a shearing time course of 12, 14, and 16 minutes.

Thank you

hamid
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