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Old 11-07-2019, 06:33 AM   #3
kmcarr
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Location: USA, Midwest

Join Date: May 2008
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Quote:
Originally Posted by samd View Post
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Nothing seemed to change in the "quality" of the DNA as I got similar gel results and quantifications. However I did get different QC results with the first good run having a nice skinny peak, and the bad runs having more of a lumpy hill around the fragment size (attached a picture below).
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Don't over interpret the differences between these two traces since they were obviously performed using different instruments. The one on the left appears to be an Advanced Analytical Fragment Analyzer and on the right an Agilent TapeStation. Due to differences in the electrophoretic matrix and run conditions the same library may appear to migrate differently on different instruments. Also note that the X axis (size scale) is much more compressed on the Fragment Analyzer relative to the TapeStation so naturally peaks will appear more compressed.
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