View Single Post
Old 03-02-2017, 05:05 AM   #3
Junior Member
Location: Moscow, Russia

Join Date: Feb 2017
Posts: 2

This is what I could extract from core lab personnel:

1- Kit or method used for library prep

Genomic DNA was extracted from tissue, BS treated, sonicated, end repaired, dA-tailed. Then standard illumina adaptors were used for PE sequencing.

2- Read length

100bases (adaptors already trimmed)

3- Library peak size


4- FastQC output for reads

sorry, I can't attach picture right now, but fastQC report is good for all reads median quality at 5' end is 30, at 3' end is ~15. And I preformed quality trimming with threshold over 15.
zubr is offline   Reply With Quote