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Old 10-14-2015, 08:53 AM   #1
Location: USA

Join Date: May 2009
Posts: 36
Default Targeted amplicon sequencing, is full overlap necessary?


Has anyone got experience running targeted amplicon libraries at less than full overlap?

We have been asked to run thunderbolts libraries that recommend 2x 250 bp to achieve full overlap but we only have access to 2x 150.

Is this a total waste of time or just not ideal?

Ideally we'd want to reach variant frequency of <<10%

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