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Old 03-23-2016, 11:51 PM   #47
Runuply
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Location: Japan

Join Date: Feb 2016
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Quote:
Originally Posted by LOH View Post
You have huge peaks at size > 1kb region, indicating your libraries are over enriched with fragments >1kb. Fragments >1kb will decrease the sequencing efficiency and is not suitable for Illumina sequencer.
Hi LOH
Could you please tell us, what should be the factors to get large size libraries sizes over 1kb. Do you think it is under-tagmentation, cell number dependent?

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Runuply
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