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Old 10-27-2016, 10:15 AM   #56
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Location: Columbus, Ohio, USA

Join Date: Jun 2016
Posts: 3

Originally Posted by seed222222 View Post
Hi maddy,
I am also trying to perform ATAC-seq on fresh cell. Did you solve the cell collection problem? Are you use trypsin or harvest cell by scraping? Thank you.
Yes, I made the ATAC-seq libraries and sent my samples for sequencing. I started with 54,000 cells and performed the TD reaction.
For collecting cells, first I discarded the media, washed cells in PBS twice. Added TryPLE to detach cells and scraped them gently in regular media. Followed by counting cells in hemocytometer.
Hope this helps. Good luck!
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