Originally posted by sunguk
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The 35bp and ~10.3kb peaks, are the spike-in size standards that Bioanalyzers use for DNA high sensitivity chips.
Normally TruSeq "PCR-Free" libraries will produce a range of product sizes similar to this. Of course, the longer products will fail to produce sequence-able clusters. They seem to be unable to compete with the shorter products.
Of course the pure-programmers probably aren't reading this sub-forum. But to any who are: Please don't require insert sizes to run your assembler/mappers! If your algorithms really need that information, figure it out! The bioanalyzer results don't give you an accurate assessment of the insert sizes, for the reasons I describe above. So asking the lab what the average size of the inserts were is not terribly useful!
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Phillip
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