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Old 05-07-2014, 10:21 PM   #2
ScottC
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Location: Monash University, Melbourne, Australia.

Join Date: Jan 2008
Posts: 246
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Yes, you can certainly synthesise your own primers if you want to do that. The kit is really only for convenience. Illumina will recommend it becase 1) that's what Illumina used when they tested the procedure and 2) they want you to buy their reagents.

You can make your own oligos and use your own PCR reagents if you wish. We use our own PCR reagents but we use the Illumina adapters, because we already have them in our lab (we use them with the rest of the Nextera XT kit when preparing genomic DNA libraries).
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