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Old 03-21-2018, 11:53 AM   #8
thermophile
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Location: CT

Join Date: Apr 2015
Posts: 243
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FAE checked over the first miseq. nothing horribly out of wack. replaced the syringe, realigned the flowcell clamp (it was at the edge of spec which he thought *could* cause some errors). I showed him a bunch of my last runs, which are weird but he can't explain them either. Running another phiX using a new lot of flowcells, it looks fine. So it's still either a flowcell issue or a machine issue. No answers.
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Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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