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Old 12-07-2018, 05:34 AM   #1
seqtechno1
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Location: U.S.

Join Date: May 2017
Posts: 21
Smile demultiplexing multiple runs with and without samples same sample sheet

Hello all,

I'm going to demultiplex a run with a sample sheet in which there are entries and adapter sequences in the sheet with no corresponding physical samples in the pool. I'm doing this for two reasons:

a. so that I don't have to modify the samplesheet and have multiple samplesheets for a project involving multiple sequencing runs (mitigate confusion)

b. I'm honestly curious how many 'reads' we will see after demultiplexing and what they represent. will they all originate from base calling error(s), or what would you call these (counts) I get after demultiplexing if, say, I get some very low number of counts for the sample which was not present?
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