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Old 09-09-2015, 12:36 AM   #24
Senior Member
Location: US

Join Date: Dec 2010
Posts: 354

Hi Brian,

when using BBsplit (also latest version) with long reads (Moleculo data) the program shreds these long reads into 500 bp pieces. It seems to me that the distributing of the reads according to reference is not working in this case. For me about 0.5% of the genomic reads are mapping to mitochondria according to the BBsplit report, but the out-files contain about 99% of the reads. Perhaps I am misinterpreting anything?

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