Thread: phred 33 or 64
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Old 04-24-2018, 06:04 AM   #4
Location: Brazil

Join Date: Aug 2015
Posts: 69

It is actually easy to spot (see the table here): in Phred + 33, capital letters are at the top end of the quality range (Phred score plus 33 gives an ASCII code for a capital letter when Phred is 32 to 42), while a capital letter would be an abysmal score in Phred +64. And in your case, you have "#" which is only valid for Phred +33 (ASCII code 35).
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