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Old 05-31-2018, 04:28 AM   #4
Location: Canada

Join Date: Jun 2013
Posts: 56

You are very welcome. Sounds like over-drying is likely your main problem. If you are worried about ethanol contamination and want to minimize drying time you can remove as much ethanol as you can from the tubes, briefly spin the tubes to collect the liquids and beads at the bottom, then move the paramagnetic beads to the side of the tube with a neodymium magnet and collect the remaining liquids with a 10-20 ul pipette tip. After this, a minute or two of drying time should allow virtually all the ethanol to evaporate and the beads should look like moist/wet clay but not cracked.
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