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RNA Purification Beads during First and Second Strand Synthesis
I'm using the Tru-seq Stranded mRNA Sample Preparation kit and I made the mistake of not removing the RNA from the RNA Purification Beads before starting the First strand synthesis and then second strand synthesis. The only step I missed was removing the beads, I added everything else and ran the incubations required for the first and second strand synthesis. Is everything okay to continue or will I have to start over?
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Today, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM