Hey guys,
I could not find a precise information of how to calculate the physical coverage from genome coverage, assuming that I have a paired end library, where both reads have an average length of 70bps and the insert size is around 200bp.
Does the definition of physical coverage include both reads?
I was guessing that it does and calculated it the following way:
Physical_Coverage=Genome_Coverage*(2*Read_Length+Insert_Size)/(2*Read_Length)
As an example:
If I know that my genome coverage is 4,
then the phyiscal coverage is 4*(2*70+200)/(2*70)=9.7
Is that correct?
Thanks in advance!
Tristan
I could not find a precise information of how to calculate the physical coverage from genome coverage, assuming that I have a paired end library, where both reads have an average length of 70bps and the insert size is around 200bp.
Does the definition of physical coverage include both reads?
I was guessing that it does and calculated it the following way:
Physical_Coverage=Genome_Coverage*(2*Read_Length+Insert_Size)/(2*Read_Length)
As an example:
If I know that my genome coverage is 4,
then the phyiscal coverage is 4*(2*70+200)/(2*70)=9.7
Is that correct?
Thanks in advance!
Tristan
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