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Old 12-14-2010, 05:10 PM   #1
Location: Detroit

Join Date: Dec 2010
Posts: 19
Default Paired End Protocol for Very Low Abundance Samples


This is my first post after sometime lurking. Utilizing what I've seen in the forum I think I have suitably adapted the standard Illumina ChIP paired end protocol to achieve our goal of generating a sequencing library from 0.3 -0.5 ng input. The changes are not surprising (reduced vol. ligation step, diluting adapters, 20 cycles PCR enrichment, etc).

Has anyone come across a reliable protocol or perhaps have any other suggests. Thanks everyone.
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