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Old 05-18-2011, 01:27 PM   #4
Location: Sweden

Join Date: Nov 2009
Posts: 83

Originally Posted by ning View Post
I think maybe you could roughly look at the mean and var of each gene. If the var/mean is much higher than 1, then you may treat them as "bilogical rep"? And if the var/mean is around 1, you may treat them as "tech rep".. Since people saw technical reps are poisson distributed. And for biological reps, the var of the poisson is overdispersed.
I'm not sure what the relationship between var and mean has to do with whether the samples are classified as biological or technical replicates. A biological replicate requires that the samples are independent. Extracting a second sample from the same individual will allow you to determine technical bias but it does not increase knowledge about how biologically reproducible a result it. Simon Andrews has posted some good answers in relation to this in other threads.
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