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Old 09-21-2015, 10:34 PM   #1
alaminos
Junior Member
 
Location: germany

Join Date: Sep 2015
Posts: 5
Default RAD-seq pool or not newbie question

Dear all,

So my question is regarding pooling individuals or not for RAD-seq.
First of all we will use 3 lanes of illumina hi seq 2500 and my project involves sequencing of bees across 18 sites. Sites that differ in terms of landscape.
So we will use RADseq for both identifying demographic parameters from neutral markers and candidate loci undergoing selection for complex habitats.
Since we have one plate and 3 lanes of illumina we could either genotype 5 individuals per population (site) or we pool about 25 individuals (thats how much I have) per population.
Which of the two ways do you think is preferred for the purpose of our study.

Thanks in advance

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