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Old 09-22-2015, 01:21 PM   #2
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Location: Eugene, OR

Join Date: May 2013
Posts: 521

I think it is difficult to get accurate SNP frequency estimates from pooled RAD data. You could identify SNPs present/absent in each pool though.

I'm not sure why your project is limited to 1 plate? Could you sequence 1 plate of individuals in each lane for 285 total? Unless you have many loci that should be possible.
Providing nextRAD genotyping and PacBio sequencing services.
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