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Old 05-06-2016, 08:06 AM   #3
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 243

what are you isolating from? I've had the most success getting large DNA out of environmental samples (bacteria and fungi, so difficult to burst cells) using the old school hot SDS and CTAB lysis followed by very gentle phenol chloroform extraction. Pipet gently-maybe even using wide bore tips. I haven't tried the minion yet, I was making fosmid libraries
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