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Old 05-06-2016, 08:06 AM   #3
thermophile
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Location: CT

Join Date: Apr 2015
Posts: 243
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what are you isolating from? I've had the most success getting large DNA out of environmental samples (bacteria and fungi, so difficult to burst cells) using the old school hot SDS and CTAB lysis followed by very gentle phenol chloroform extraction. Pipet gently-maybe even using wide bore tips. I haven't tried the minion yet, I was making fosmid libraries
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