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Old 02-05-2020, 09:25 AM   #21
BrianJames
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Location: San Diego

Join Date: May 2010
Posts: 13
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Hi ShyN,
What ribo depletion are you using? We've had good luck with the NEB reagent, and it is a pretty easy protocol. We've been using the EP motion for our polyA libraries with the NEB Ultra II directional kit (1/2 reactions) with very good results. We do some steps off deck, but all of the cleanups are on the instrument.
Hope that helps.
Brian
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