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Old 03-25-2020, 12:19 PM   #1
Junior Member
Location: France

Join Date: Mar 2020
Posts: 2
Default Indeterminate nucleotides ?

Hello !
I am a French student and I am very interested in DNA sequencing.
I downloaded the sequenced human genome (in Fasta format) from the "U.S. National Library of Medicine" to study it.
Approximately 7% of the nucleotides are replaced by the letter N, which means that it's Adenine, Cytosine, Guanine OR Thymine, so it's indeterminate.

Could someone explain to me how it's possible to have indeterminate nucleotides?
Nobody answered me on the French forums or on the less specific American forums.

Thank you in advance
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