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Old 02-14-2014, 08:12 AM   #2
MissDNA
Senior Member
 
Location: Brazil

Join Date: Nov 2010
Posts: 146
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I really hope you guys have opted for MiSeq.

We have had the Ion Proton for a bit over a year now and so far nothing really good came out of it.

Answering your questions:
It is very easy to use, especially when you compare with 454.
We have used for whole genome, metagenome and transcriptome so far. Our best run reached only 9.4 Gb, mean read length 120 bp. We have had entire runs where not a single base reached Q30! Quality of the reads is really, really poor.
So yeah, real-world performance is not corresponding with published specs so far and we are not seeing any improvement.
We are not glad we got it, we wish we had gone with illumina!

Last edited by MissDNA; 02-14-2014 at 08:17 AM.
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