Hi,
I have a couple of questions regarding the input files for tophat.I am aligning RNA-Seq reads with canfam2(dog) genome.
1.Do I have to create an index file for the RNA-seq reads like creating an index file for canfam2 genome or should I just use the fastq file directly as input.
Thanks,
Joji
I have a couple of questions regarding the input files for tophat.I am aligning RNA-Seq reads with canfam2(dog) genome.
1.Do I have to create an index file for the RNA-seq reads like creating an index file for canfam2 genome or should I just use the fastq file directly as input.
Thanks,
Joji